News: Aug 15, 2016
Plants, bacteria and fungi react to light with light-sensitive proteins. Scientists from the University of Gothenburg and their Finnish colleagues from University of Jyväskylä have now determined the inner workings of one of these proteins. The results have been published in the most recent issue of Science Advances.
The investigated proteins are called “phytochromes”. They consist of thousands of atoms and can be thought of as tiny, microscopic machines. These proteins are found in all plant leaves, many bacteria and fungi. The proteins inform the cell whether it is day or night or whether it is cloudy or sunny.
“Phytochrome proteins are the eyes of plants and in many bacteria. We have now discovered how bacterial phytochromes work at the molecular level,” explains Sebastian Westenhoff at the Department of Chemistry and Molecular Biology at the University of Gothenburg.
Efficient photosynthesis requires that leaves are exposed to the sun. For this, the plants have to grow towards sunlight and phytochrome proteins control this process. Similarly, bacteria use phytochromes to move to spots where they can survive better. The proteins detect the light and signal to the plant cell how much light is available.
“Each time a phytochrome protein absorbs light, it deforms in a well-orchestrated series of structural changes. We already discovered an early structural change two years ago. Back then we used a shortened phytochrome. In the meantime we have advanced our experimental methods and could now study a full-length protein with a biological activator unit, called histidine kinase. This revealed the change in the final stage of the process.” says Sebastian Westenhoff.
The discovery increases our understanding of how phytochromes work. This enables modification of the proteins, for example to increase crop yield. However, the new knowledge is also crucial for another technology, where scientists engineer light sensitive proteins to control organism by light. Potentially such artificial proteins can be used to release drugs at specific spots in out body, for example in cancer cells.
“Proteins are molecular nanomachines, which control most of what we see in Nature. Deciphering the structure of proteins is key to understanding how the machines work. This knowledge can also be used to modify or construct new proteins, with custom-built functions,” says Sebastian Westenhoff.
The project was carried out as a collaboration between two groups at the University of Gothenburg and the University of Jyväskylä in Finland. However, more collaboration was needed and the data for the study was recorded at experimental facilities in France, Switzerland, Finland, and the US.
“Numerous data sets had to be recoded and evaluated until a reliable and complete result was obtained.” says Sebastian Westenhoff, “but I think that all the hard work was worth it, because we now understand better how plants and bacteria see light.”
Sebastian Westenhoff, Department for Chemistry and Molecular Biology
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Originally published on: science.gu.se