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Postdoctoral scholarship: Impact of peroxiredoxins on protein synthesis

Nyhet: 2018-03-13

Project description: This Post-Doctoral stipend is intended for the recipient to learn more about molecular details of how the peroxiredoxin Tsa1 slows down aging using yeast replicative aging as a model. More specifically you will further study mechanisms connecting peroxiredoxin-mediated H2O2-signaling to the regulation of protein biogenesis.

Competences: We seek a talented and dedicated postdoctoral fellow eager to pursue a career in microbiology/molecular biology. You should be open-minded and have a genuine interest in Science. Abilities to work both independently and in a team as well as to communicate well in English are crucial. The ideal candidate should have documented research experience in genetics, molecular biology and/or in vivo biochemistry. Experience with yeast, studies of aging, protein biogenesis and/or metabolism are considered an advantage. Candidates should either hold a PhD degree or obtain it before the start of the project.

More about us and the project: Mikael Molin’s (MM’s) lab works on the impact of H2O2-signaling via peroxiredoxins on organismal physiology and aging. Peroxiredoxins are conserved antioxidants and signaling enzymes, which have received increased attention lately because of a conserved function in slowing down the rate of aging in yeast and in multicellular organisms (Molin M et al Int J Cell Biol 2014, Nyström T et al Genes Dev 2012). Notably, peroxiredoxin activity has been shown to be stimulated upon both caloric restriction (Molin M et al, Mol Cell, 2011) and treatment with the anti-glycemic drug metformin, to slow down the rate of aging of yeast and worms, respectively, suggesting that peroxiredoxins are the long sought target enzymes explaining longevity upon dietary restrictions and that they can be targeted therapeutically. However, their molecular functions still remain poorly understood.

MM’s research has, furthermore, shown that 1) low levels of H2O2 are not detrimental to yeast cells but rather slow down aging via the peroxiredoxin Tsa1 (Goulev Y et al, eLIFE, 2017), 2) Tsa1 is a potent gerontogene dramatically slowing down aging upon just a moderate 2-fold overexpression, 3) the anti-aging effect of Tsa1 requires normal levels of Hsp70 proteins and normal proteasome activity (Hanzén S et al, Cell, 2016).

Functions of Hsp70 proteins include preventing proteins from aggregating and/or facilitating their disaggregation or degradation by the proteasome. Interestingly, however, our data suggest that this may not be the primary function of peroxiredoxins that inhibits aging. Instead, our data point to that Hsp70-dependent signaling functions impinging on metabolism and/or protein biogenesis are crucial to slow down aging. The recipient of this Post-Doctoral stipend will study the role of Tsa1 in the regulation of protein biogenesis further using eg sucrose gradient centrifugation (polysome analysis) and ribosomal sequencing. Other techniques used will be yeast genetic analysis, in vivo cysteine redox-analyses, analysis of protein ubiquitinylation, proteasome activity and proteasomal degradation.

To better understand peroxiredoxin-Hsp70 interactions MM’s group collaborates with Dr Björn Burmann at CMB. Björn Burmann’s group investigates macromolecular protein machines underlying essential cellular functions, e.g. protein quality control and DNA-repair processes, through biophysical and structural biology approaches e.g. high-resolution Nuclear Magnetic Resonance (NMR). This Post-Doctoral stipend is thus intended to provide complementary approaches to address these questions mostly using in vivo approaches.

Research environment: Yeast genetics and cell biology is a strong research area at the department of Chemistry and Molecular Biology (CMB) and the participating research groups are highly interactive and share technology platforms for high-throughput genetics and cell biology. Thus CMB provides an excellent environment for successful interdisciplinary research in life science and an outstanding training ground for students and Post-Docs in molecular biology.

Dnr: E 2018/191
Scholarship period: The scholarship covers a period of 12 months with a possibility of prolongation up to a maximum of 24 months.
Preliminary start date: 2018-05-15
Supervisor/contact person:
Mikael Molin, mikael.molin@cmb.gu.se, 031-786 2577
Björn Burmann, bjorn.marcus.burmann@gu.se, 031-786 3923

• To be eligible for a post-doc scholarship at the recipient must hold a PhD degree within a relevant field. The applicant must not have been employed at University of Gothenburg in the past two years.
• Please specify experiences, knowledge or interests necessary for taking part as a postdoc in this project. Remember that a scholarship is not an employment and the qualifications necessary might differ from what you could ask for from an employee.
Written application, including reference number, is to be sent via e-mail to the supervisor and must include the following:
• CV
• Personal letter stating the reasons why the study suits the applicant (maximum one page)
• List of publications
• References (2)

Application deadline: 2018-04-20

Information regarding scholarships:
• Scholarships do not give rise to sickness benefits, compensation from the Social Insurance Office or retirement pension.
• The scholarship sum is paid out quarterly
• Scholarships are tax-exempt
• A scholarship holder cannot be hired after the scholarship period due to tax reasons.


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