Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

John Fletcher

Senior lecturer

John Fletcher
Senior lecturer
john.fletcher@chem.gu.se
+46 31 786 9105
0766-229105

Room number: 4032
Postal Address: Kemigården 4, 412 96 Göteborg
Visiting Address: Kemigården 4 , 412 96 Göteborg


Department of Chemistry & Molecular Biology (More Information)
Box 462
405 30 Göteborg
www.cmb.gu.se
inst.kmb@cmb.gu.se
Fax: +46 31 786 2599
Visiting Address: Medicinaregatan 9 C , 405 30 Göteborg

About John Fletcher

 

My groups research is focused on the development and application of imaging mass spectrometry for biological analysis. Mass spectrometry provides molecular specific chemical information without the need to incorporate exogenous labels. When used in an imaging modality this chemical precision is combined with precise spatial distribution. Secondary ion mass spectrometry (SIMS) uses a focused ion beam to probe the surface of a sample and the nature of the ion beam fired at the sample can ultimately determine what chemical species are detected in the mass spectrometer.
As there is a constant demand for more signal at higher resolution and so ion beam development is an active area of research particularly using new gas cluster ion beam technology. For biological sample analysis ensuring that the data generated is representative of the "live" sample means that methods to prepare and analyse biological specimen in the vacuum environment required for imaging SIMS are critical.
Combining the new ion beams with optimal sample preperation provides a route to identifying lipidomic changes on a sub-cellular scale, potentially in 3D, in order elucidate the underlying chemical processes involved in disease progression and treatment.

 

Current PhD students

Marwa Munem

Kelly Dimovska Nilsson 

Teaching and Departmental Responsibilities

KEM070 - Basic Analytical Chemistry

KEM081 - Advanced Analytical Chemistry

KEA310 - Introduction to Analytical Chemistry

Analytical Chemistry Project Contact

Coordinator of Chemistry Masters Program

Departmental Strategy Board Member

 

 

Latest publications

Cholesterol Alters the Dynamics of Release in Protein Independent Cell Models for Exocytosis
Neda Najafinobar, Lisa J. Mellander, Michael E. Kurczy, Johan Dunevall, Tina B. Angerer et al.
Scientific Reports, Journal article 2016
Journal article

Showing 41 - 50 of 80

2013

The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications.
Paul Bassan, Joe Lee, Ashwin Sachdeva, Juliana Pissardini, Konrad M Dorling et al.
The Analyst, Journal article 2013
Journal article

2012

A Comparative Study of Secondary Ion Emission from Water Ice under Ion Bombardment by Au(+), Au(3)(+), and C(60)(+)
Xavier A Conlan, John S. Fletcher, Nicholas P Lockyer, John C Vickerman
Journal of Physical Chemistry C, Journal article 2012
Journal article

2011

Top-down approach to studying biological components using ToF-SIMS
Alan M. Piwowar, John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Surface and Interface Analysis, Journal article 2011
Journal article

Investigating the effect of temperature on depth profiles of biological material using ToF‐SIMS
Alan M. Piwowar, John S. Fletcher, Nicholas Lockyer, John Vickerman
Surface and Interface Analysis, Journal article 2011
Journal article

Exploring subcellular imaging on the buncher-ToF J105 3D chemical imager
S. Rabbani, John S. Fletcher, N. P. Lockyer, J. C. Vickerman
Surface and Interface Analysis, Conference paper 2011
Conference paper

DEVELOPMENTS IN MOLECULAR SIMS DEPTH PROFILING AND 3D IMAGING OF BIOLOGICAL SYSTEMS USING POLYATOMIC PRIMARY IONS
John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Mass Spectrometry Reviews, Review article 2011
Review article

A new time-of-flight SIMS instrument for 3D imaging and analysis
Rowland Hill, Paul Blenkinsopp, Stephen Thompson, John Vickerman, John S. Fletcher
Surface and Interface Analysis, Conference paper 2011
Conference paper

Molecular SIMS imaging; spatial resolution and molecular sensitivity: Have we reached the end of the road? Is there light at the end of the tunnel?
John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Surface and Interface Analysis, Conference paper 2011
Conference paper

TOF-SIMS with Argon Gas Cluster Ion Beams: A Comparison with C(60)(+)
Sadia Rabbani, Andrew M. Barber, John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Analytical Chemistry, Journal article 2011
Journal article

Three-dimensional mass spectral imaging of HeLa-M cells - sample preparation, data interpretation and visualisation
John S. Fletcher, Sadia Rabbani, Alex Henderson, Nicholas P. Lockyer, John C. Vickerman
Rapid Communications in Mass Spectrometry, Journal article 2011
Journal article

Showing 41 - 50 of 80

Page Manager: Katleen Burm|Last update: 9/20/2013
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?