Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

John Fletcher

Senior lecturer

John Fletcher
Senior lecturer
john.fletcher@gu.se
+46 31 786 9105
0766-229105

Room number: 4032
Postal Address: Kemigården 4, 412 96 Göteborg
Visiting Address: Kemigården 4 , 412 96 Göteborg


Department of Chemistry & Molecular Biology (More Information)
Box 462
405 30 Göteborg
www.cmb.gu.se
inst.kmb@cmb.gu.se
Fax: +46 31 786 2599
Visiting Address: Medicinaregatan 9 C , 413 90 Göteborg

About John Fletcher

 

My groups research is focused on the development and application of imaging mass spectrometry for biological analysis. Mass spectrometry provides molecular specific chemical information without the need to incorporate exogenous labels. When used in an imaging modality this chemical precision is combined with precise spatial distribution. Secondary ion mass spectrometry (SIMS) uses a focused ion beam to probe the surface of a sample and the nature of the ion beam fired at the sample can ultimately determine what chemical species are detected in the mass spectrometer.
As there is a constant demand for more signal at higher resolution and so ion beam development is an active area of research particularly using new gas cluster ion beam technology. For biological sample analysis ensuring that the data generated is representative of the "live" sample means that methods to prepare and analyse biological specimen in the vacuum environment required for imaging SIMS are critical.
Combining the new ion beams with optimal sample preperation provides a route to identifying lipidomic changes on a sub-cellular scale, potentially in 3D, in order elucidate the underlying chemical processes involved in disease progression and treatment.

 

Current PhD students

Marwa Munem

Kelly Dimovska Nilsson 

Teaching and Departmental Responsibilities

KEM070 - Basic Analytical Chemistry

KEM081 - Advanced Analytical Chemistry

KEA310 - Introduction to Analytical Chemistry

Analytical Chemistry Project Contact

Coordinator of Chemistry Masters Program

Departmental Strategy Board Member

 

 

Latest publications

Mass Spectrometry Imaging and Integration with Other Imaging Modalities for Greater Molecular Understanding of Biological Tissues
T. P. Siegel, G. Hamm, J. Bunch, J. Cappell, John S. Fletcher et al.
Molecular Imaging and Biology, Review article 2018
Review article

Cholesterol Alters the Dynamics of Release in Protein Independent Cell Models for Exocytosis
Neda Najafinobar, Lisa J. Mellander, Michael E. Kurczy, Johan Dunevall, Tina B. Angerer et al.
Scientific Reports, Journal article 2016
Journal article

Showing 51 - 60 of 82

2011

TOF-SIMS with Argon Gas Cluster Ion Beams: A Comparison with C(60)(+)
Sadia Rabbani, Andrew M. Barber, John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Analytical Chemistry, Journal article 2011
Journal article

Three-dimensional mass spectral imaging of HeLa-M cells - sample preparation, data interpretation and visualisation
John S. Fletcher, Sadia Rabbani, Alex Henderson, Nicholas P. Lockyer, John C. Vickerman
Rapid Communications in Mass Spectrometry, Journal article 2011
Journal article

Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry.
John S. Fletcher, John C Vickerman, Nicholas Winograd
Current opinion in chemical biology, Review article 2011
Review article

2010

Time of Flight Mass Spectrometry Imaging of Samples Fractured In Situ with a Spring-Loaded Trap System.
Ingela Lanekoff, Michael E. Kurczy, Rowland Hill, John S. Fletcher, John C Vickerman et al.
Analytical chemistry, Journal article 2010
Journal article

A new SIMS paradigm for 2D and 3D molecular imaging of bio-systems
John S. Fletcher, John C. Vickerman
Analytical and Bioanalytical Chemistry, Review article 2010
Review article

Effects of Cryogenic Sample Analysis on Molecular Depth Profiles with TOF-Secondary Ion Mass Spectrometry
Alan M. Piwowar, John S. Fletcher, Jeanette Kordys, Nicholas P. Lockyer, Nicholas Winograd et al.
Analytical Chemistry, Journal article 2010
Journal article

2 and 3D TOF-SIMS Imaging for Biological Analysis
John S. Fletcher
Detection of Biological Agents For the Prevention of Bioterrorism, Chapter in book 2010
Chapter in book

2009

A Comparative Study of Secondary Ion Emission from Water Ice under Ion Bombardment by Au+, Au3+, and C60+
Conlan Xavier, John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Journal of Physical Chemistry C, Journal article 2009
Journal article

Explanatory multivariate analysis of ToF-SIMS spectra for the discrimination of bacterial isolates
Seetharaman Vaidyanathan, John S. Fletcher, Roger M. Jarvis, Alex Henderson, Nicholas P. Lockyer et al.
Analyst, Journal article 2009
Journal article

A comparison of PCA and MAF for ToF-SIMS image interpretation
Alex Henderson, John S. Fletcher, John C. Vickerman
Surface and Interface Analysis, Journal article 2009
Journal article

Showing 51 - 60 of 82

Page Manager: Katleen Burm|Last update: 9/20/2013
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?