Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

John Fletcher

Senior lecturer

John Fletcher
Senior lecturer
john.fletcher@gu.se
+46 31 786 9105
0766-229105

Room number: 4032
Postal Address: Kemigården 4, 412 96 Göteborg
Visiting Address: Kemigården 4 , 412 96 Göteborg


Department of Chemistry & Molecular Biology (More Information)
Box 462
405 30 Göteborg
www.cmb.gu.se
inst.kmb@cmb.gu.se
Fax: +46 31 786 2599
Visiting Address: Medicinaregatan 9 C , 413 90 Göteborg

About John Fletcher

 

My groups research is focused on the development and application of imaging mass spectrometry for biological analysis. Mass spectrometry provides molecular specific chemical information without the need to incorporate exogenous labels. When used in an imaging modality this chemical precision is combined with precise spatial distribution. Secondary ion mass spectrometry (SIMS) uses a focused ion beam to probe the surface of a sample and the nature of the ion beam fired at the sample can ultimately determine what chemical species are detected in the mass spectrometer.
As there is a constant demand for more signal at higher resolution and so ion beam development is an active area of research particularly using new gas cluster ion beam technology. For biological sample analysis ensuring that the data generated is representative of the "live" sample means that methods to prepare and analyse biological specimen in the vacuum environment required for imaging SIMS are critical.
Combining the new ion beams with optimal sample preperation provides a route to identifying lipidomic changes on a sub-cellular scale, potentially in 3D, in order elucidate the underlying chemical processes involved in disease progression and treatment.

 

Current PhD students

Marwa Munem

Kelly Dimovska Nilsson 

Teaching and Departmental Responsibilities

KEM070 - Basic Analytical Chemistry

KEM081 - Advanced Analytical Chemistry

KEA310 - Introduction to Analytical Chemistry

Analytical Chemistry Project Contact

Coordinator of Chemistry Masters Program

Departmental Strategy Board Member

 

 

Latest publications

Mass Spectrometry Imaging and Integration with Other Imaging Modalities for Greater Molecular Understanding of Biological Tissues
T. P. Siegel, G. Hamm, J. Bunch, J. Cappell, John S. Fletcher et al.
Molecular Imaging and Biology, Review article 2018
Review article

Cholesterol Alters the Dynamics of Release in Protein Independent Cell Models for Exocytosis
Neda Najafinobar, Lisa J. Mellander, Michael E. Kurczy, Johan Dunevall, Tina B. Angerer et al.
Scientific Reports, Journal article 2016
Journal article

Showing 61 - 70 of 82

2009

Cellular imaging with secondary ion mass spectrometry
John S. Fletcher
Analyst, Review article 2009
Review article

2008

Exploratory analysis of TOF-SIMS data from biological surfaces
Seetharaman Vaidyanathan, John S. Fletcher, Alex Henderson, Nicholas P. Lockyer, John C. Vickerman
Applied Surface Science, Conference paper 2008
Conference paper

TOF-SIMS investigation of Streptomyces coelicolor, a mycelial bacterium
Seetharaman Vaidyanathan, John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Applied Surface Science, Journal article 2008
Journal article

Substrate effects on the analysis of biomolecular layers using Au+, Au3+ and C60+ bombardments
Jeanette Kordys, John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Applied Surface Science, Journal article 2008
Journal article

Subsurface Biomolecular Imaging of Streptomyces coelicolor Using Secondary Ion Mass Spectrometry
Seetharaman Vaidyanathan, John S. Fletcher, Roy Goodacre, Nicholas P. Lockyer, Jason Micklefield et al.
Analytical Chemistry, Journal article 2008
Journal article

A New Dynamic in Mass Spectral Imaging of Single Biological Cells
John S. Fletcher, Sadia Rabbani, Alex Henderson, Paul Blenkinsopp, Steve P. Thompson et al.
Analytical Chemistry, Journal article 2008
Journal article

Uncovering new challenges in bio-analysis with ToF-SIMS
John S. Fletcher, Alex Henderson, Gregory X. Biddulph, Seetharaman Vaidyanathan, Nicholas P. Lockyer et al.
Applied Surface Science, Conference paper 2008
Conference paper

2007

TOF-SIMS 3D biomolecular imaging of Xenopus laevis oocytes using buckminsterfullerene (C-60) primary ions
John S. Fletcher, Nicholas P. Lockyer, Seetharaman Vaidyanathan, John C. Vickerman
Analytical Chemistry, Journal article 2007
Journal article

Properties of C-84 and C24H12 molecular ion sources for routine TOF-SIMS analysis
Gregory X. Biddulph, Alan M. Piwowar, John S. Fletcher, Nicholas P. Lockyer, John C. Vickerman
Analytical Chemistry, Journal article 2007
Journal article

2006

TOF-SIMS analysis using C-60- effect of impact energy on yield and damage
John S. Fletcher, X. A. Conlan, E. A. Jones, G. Biddulph, N. P. Lockyer et al.
Analytical Chemistry, Journal article 2006
Journal article

Showing 61 - 70 of 82

Page Manager: Katleen Burm|Last update: 9/20/2013
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?